Electrophoretic Mobility Distributions of Single-Strain Microbial Populations

314 Electrophoretic Mobility and Agglutinability

I t has been recognized since 1954 (1) that the red cells of mice of some strains, notably A, can be specifically agglutinated more readily by anti-H-2 sera than those of others. This difference has been shown to apply to several 1t-2 antigens (2) and has been ascribed to a different cell concentration of antigenic determinants (3). Experience shows, however, that nonspecific agglutinability is...

Electrophoretic Mobility Shift Assay (EMSA).

Transcriptional regulation of gene expression is controlled through the binding of sequence-specific DNA-binding proteins (transcription factors) to the regulatory regions of genes. The exact gene expression program of a cell is determined by the spectrum of transcription factors present with the nucleus of a cell. The presence of these factors is dependent upon the cell type being examined and...

Electrophoretic Techniques in Microbial Ecology

Electrophoretic mobility of microsomes from rat liver.

The electrophoretic mobilities of rough and smooth microsomes were studied using free electrophoresis in a sucrose gradient. Rough microsomes have a higher net negative surface charge but removal of the ribosomes decreases their mobility to that of smooth microsomes. Treatment with neuraminidase and phospholipases C and D does not affect the mobility of total smooth microsomes, but this mobilit...

Vizardous: interactive analysis of microbial populations with single cell resolution

MOTIVATION Single cell time-lapse microscopy is a powerful method for investigating heterogeneous cell behavior. Advances in microfluidic lab-on-a-chip technologies and live-cell imaging render the parallel observation of the development of individual cells in hundreds of populations possible. While image analysis tools are available for cell detection and tracking, biologists are still confron...